Optical Sensors Based on Whispering Gallery Modes in Fluorescent Microbeads: Response to Specific Interactions
نویسندگان
چکیده
Whispering gallery modes (WGMs) in surface-fixated fluorescent polystyrene microbeads are studied in view of their capability of sensing the formation of biochemical adsorption layers on their outer surface with the well-established biotin-streptavidin specific binding as the model system. Three different methods for analysis of the observed shifts in the WGM wavelength positions are applied and used to quantify the adsorbed mass densities, which are then compared with the results of a comparative surface plasmon resonance (SPR) study.
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Optical Sensors Based on Whispering Gallery Modes in Fluorescent Microbeads: Size Dependence and Influence of Substrate
Whispering gallery modes in surface-fixated fluorescent polystyrene microbeads are studied in view of their capability of sensing changes in the refractive index of the beads' environment by exposing them to water/glycerol mixtures of varying composition. The mode positions are analyzed by simultaneous fitting for mode number, bead radius, and environmental index. Down to a diameter of 8 μm, th...
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